| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Leptospira biflexa | TTGGGTGCATCGGTTCACA | GCCAACAACATCTGCCGTG | 59.85 | 60.08 | 296 | 104 |
100.00%
|
100.00%
|
| Primer 522 | Leptospira biflexa | AATCGGGTCCATTCCCTGC | TCCATTCCTTGTCGCTTCCTC | 59.77 | 60.07 | 188 | 104 |
100.00%
|
100.00%
|
| Primer 523 | Leptospira biflexa | TCGATCATTCGCCTCCGAG | ACTGGAACTGATGAGGAGTGG | 59.35 | 59.09 | 168 | 104 |
100.00%
|
100.00%
|
| Primer 524 | Leptospira biflexa | ACTTGCCGTGAAGCAGCT | GCAGTCATAAACCCCAACGC | 59.89 | 59.83 | 165 | 103 |
100.00%
|
100.00%
|
| Primer 525 | Leptospira biflexa | TCCAGAAGTGTTTTCCGCAGA | CGTGCGTTTCCAAACCACA | 59.86 | 59.57 | 172 | 103 |
100.00%
|
100.00%
|
| Primer 526 | Leptospira biflexa | ACGAGTCGGCACAAGCTT | GAATCTGCCACCGGTTTGG | 59.58 | 59.12 | 232 | 103 |
100.00%
|
100.00%
|
| Primer 527 | Leptospira biflexa | TGCCGAAGTCGCCAAAGA | TGGTGACAGGTTGGATCGAG | 59.58 | 59.39 | 230 | 103 |
100.00%
|
100.00%
|
| Primer 528 | Leptospira biflexa | TGCCGAAGTCGCCAAAGA | GGTGACAGGTTGGATCGAGA | 59.58 | 59.10 | 229 | 103 |
100.00%
|
100.00%
|
| Primer 529 | Leptospira biflexa | TGCCGAAGTCGCCAAAGA | TTGGTGACAGGTTGGATCGAG | 59.58 | 60.00 | 231 | 103 |
100.00%
|
100.00%
|
| Primer 530 | Leptospira biflexa | TGCCGAAGTCGCCAAAGA | GGTGACAGGTTGGATCGAGAG | 59.58 | 60.13 | 229 | 103 |
100.00%
|
100.00%
|